An altered CD8⁺ T cell epitope of insulin prevents type 1 diabetes in humanized NOD mice.

ABSTRACT: Autoreactive CD8⁺ T cells, which play an indispensable role in β cell destruction, represent an emerging target for the prevention of type 1 diabetes (T1D). Altered peptide ligands (APLs) can efficiently induce antigen-specific T cells anergy, apoptosis or shifts in the immune response. Here, we found that HLA-A*0201-restricted CD8⁺ T cell responses against a primary β-cell autoantigen insulin epitope InsB1_5-14 were present in both NOD.β2m^null.HHD NOD mice and T1D patients. We generated several APL candidates for InsB1_5-14 by residue substitution at the p6 position. Only H6F exhibited an inhibitory effect on mInsB1_5-14-specific CD8⁺ T cell responses in vitro. H6F treatment significantly reduc

SUBMITTER: Zhang M 

PROVIDER: S-EPMC6804845 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature