Transcription of the Subtilase Cytotoxin Gene subAB₁ in Shiga Toxin-Producing Escherichia coli Is Dependent on hfq and hns.

ABSTRACT: Certain foodborne Shiga toxin-producing Escherichia coli (STEC) strains carry genes encoding the subtilase cytotoxin (SubAB). Although the mode of action of SubAB is under intensive investigation, information about the regulation of subAB gene expression is currently not available. In this study, we investigated the regulation of the chromosomal subAB₁ gene in laboratory E. coli strain DH5α and STEC O113:H21 strain TS18/08 using a luciferase reporter gene assay. Special emphasis was given to the role of the global regulatory protein genes hfq and hns in subAB₁ promoter activity. Subsequently, quantitative real-time PCR was performed to analyze the expression of Shiga toxin 2a (Stx2a), SubAB1, and cytolethal distending t

SUBMITTER: Heinisch L 

PROVIDER: S-EPMC6805076 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature