Using an Endogenous CRISPR-Cas System for Genome Editing in the Human Pathogen Clostridium difficile.

ABSTRACT: The human enteropathogen Clostridium difficile constitutes a key public health issue in industrialized countries. Many aspects of C. difficile pathophysiology and adaptation inside the host remain poorly understood. We have recently reported that this bacterium possesses an active CRISPR-Cas system of subtype I-B for defense against phages and other mobile genetic elements that could contribute to its success during infection. In this paper, we demonstrate that redirecting this endogenous CRISPR-Cas system toward autoimmunity allows efficient genome editing in C. difficile We provide a detailed description of this newly developed approach and show, as a proof of principle, its efficient application for deletion of a specific gene in reference strain 630Δerm and

SUBMITTER: Maikova A 

PROVIDER: S-EPMC6805081 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature