Neutrophil azurophilic granule exocytosis is primed by TNF-α and partially regulated by NADPH oxidase.

ABSTRACT: Neutrophil (polymorphonuclear leukocyte) activation with release of granule contents plays an important role in the pathogenesis of acute lung injury, prompting clinical trials of inhibitors of neutrophil elastase. Despite mounting evidence for neutrophil-mediated host tissue damage in a variety of disease processes, mechanisms regulating azurophilic granule exocytosis at the plasma membrane, and thus release of elastase and other proteases, are poorly characterized. We hypothesized that azurophilic granule exocytosis would be enhanced under priming conditions similar to those seen during acute inflammatory events and during chronic inflammatory disease, and selected the cytokine TNF-α to model this in vitro. Neutrophils stimulated with TNF-α alone elicited intracellular reactive oxygen sp

SUBMITTER: Potera RM 

PROVIDER: S-EPMC6805152 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature