Locally renewing resident synovial macrophages provide a protective barrier for the joint.

ABSTRACT: Macrophages are considered to contribute to chronic inflammatory diseases such as rheumatoid arthritis¹. However, both the exact origin and the role of macrophages in inflammatory joint disease remain unclear. Here we use fate-mapping approaches in conjunction with three-dimensional light-sheet fluorescence microscopy and single-cell RNA sequencing to perform a comprehensive spatiotemporal analysis of the composition, origin and differentiation of subsets of macrophages within healthy and inflamed joints, and study the roles of these macrophages during arthritis. We find that dynamic membrane-like structures, consisting of a distinct population of CX₃CR1⁺ tissue-resident macrophages, form an internal immunological barrier at the synovial lining and physical

SUBMITTER: Culemann S 

PROVIDER: S-EPMC6805223 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature