Mutation of CarO participates in drug resistance in imipenem-resistant Acinetobacter baumannii.

ABSTRACT:

Objective

Acinetobacter baumannii has become an important problem because of the high drug resistance rate. The aim of this study was to assess the antimicrobial resistance profile and explore the role of membrane porin in imipenem resistance of A baumannii.

Methods

A total of 63 isolates of imipenem-resistant A baumannii (IRAB) and 21 of imipenem-sensitive A baumannii (ISAB) were collected. Susceptibility testing to 16 kinds of antimicrobial agents was conducted by K-B method. PCR technique was used to detect carO and oprD genes, and sequencing was performed to compare the sequence between IRAB and ISAB. Three-dimensional structure model of CarO protein was established.

Results

While ISAB isolates presented sensitive to most classes of antibiotics, isolates of IRAB d

SUBMITTER: Zhu LJ 

PROVIDER: S-EPMC6805298 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature