Genome rearrangements induce biofilm formation in Escherichia coli C - an old model organism with a new application in biofilm research.

ABSTRACT:

Background

Escherichia coli C forms more robust biofilms than other laboratory strains. Biofilm formation and cell aggregation under a high shear force depend on temperature and salt concentrations. It is the last of five E. coli strains (C, K12, B, W, Crooks) designated as safe for laboratory purposes whose genome has not been sequenced.

Results

Here we present the complete genomic sequence of this strain in which we utilized both long-read PacBio-based sequencing and high resolution optical mapping to confirm a large inversion in comparison to the other laboratory strains. Notably, DNA sequence comparison revealed the absence of several genes thought to be involved in biofilm formation, including antigen 43, waaSBOJYZUL for lipopolysaccharide (LPS) synthesis, and cpsB for

SUBMITTER: Krol JE 

PROVIDER: S-EPMC6805351 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature