In silico-guided engineering of Pseudomonas putida towards growth under micro-oxic conditions.

ABSTRACT:

Background

Pseudomonas putida is a metabolically versatile, genetically accessible, and stress-robust species with outstanding potential to be used as a workhorse for industrial applications. While industry recognises the importance of robustness under micro-oxic conditions for a stable production process, the obligate aerobic nature of P. putida, attributed to its inability to produce sufficient ATP and maintain its redox balance without molecular oxygen, severely limits its use for biotechnology applications.

Results

Here, a combination of genome-scale metabolic modelling and comparative genomics is used to pinpoint essential [Formula: see text]-dependent processes. These explain the inability of the strain to grow under anoxic conditions: a deficient ATP generation and an

SUBMITTER: Kampers LFC 

PROVIDER: S-EPMC6805499 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature