Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging.

ABSTRACT:

Background

Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the zebrafish Danio rerio in which developing tissues and organs can be easily observed and imaged using fluorescent proteins have become very popular. One limiting factor however is the acquisition of a sufficient amount of data, in standardized and reproducible conditions, to allow robust quantitative analysis. One way to improve this is by developing mounting methods to increase the number of embryos that can be imaged simultaneously in near-to-identical orientation.

Results

Here we present an improved mounting method allowing semi-automated and high-content ima

SUBMITTER: Kleinhans DS 

PROVIDER: S-EPMC6805687 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature