An Improved PCR-RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene.

ABSTRACT:

Background

In recent research, it has been shown that rs2289487 within the PLIN1 gene has different variants that have been associated with obesity, type 2 diabetes, and other diseases. However, the isochizomers such as the BsmI enzyme required for detection of this polymorphism through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method are expensive. In this study, we aimed to explore a novel PCR-RFLP method for identifying the single-nucleotide polymorphism (SNP) of rs2289487 of PLIN1 gene.

Methods

A new restriction enzyme site was created through created restriction site PCR. In the forward primer, a deoxynucleotide A was substituted with C, and after PCR a new restriction enzyme site for BstUI was introduced into the PCR products. A tota

SUBMITTER: Feng X 

PROVIDER: S-EPMC6806717 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature