Developing and Evaluating the HRM Technique for Identifying Cytochrome P450 2D6 Polymorphisms.

ABSTRACT:

Background

Cytochrome P450 2D6 is one of the important enzymes involved in the metabolism of many widely used drugs. Genetic polymorphisms of CYP2D6 can affect its activity. Therefore, an efficient method for identifying CYP2D6 polymorphisms is clinically important.

Methods

We developed a high-resolution melting (HRM) analysis to investigate CYP2D6 polymorphisms. Genomic DNA was extracted from peripheral blood samples from 71 healthy individuals. All nine exons of the CYP2D6 gene were sequenced before screening by HRM analysis. This method can detect the most genotypes (*1, *2, *4, *10, *14, *21 *39, and *41) of CYP2D6 in Chinese.

Results

All samples were successfully genotyped. The four most common mutant CYP2D6 alleles (*1, *2, *10, and *41) can be genotyped. The si

SUBMITTER: Lu HC 

PROVIDER: S-EPMC6806731 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature