A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes.

ABSTRACT:

Background

Duffy blood group genotyping is useful to ensure transfusion safety and determine the association of Duffy blood group polymorphism with diseases, and therefore has its clinical significance. In order to improve the existing methods for genotyping of Duffy blood group, which normally require post-PCR manipulation, a new method was developed by using 5'-nuclease assay (NA) with TaqMan minor groove binding (MGB) probes.

Methods

Primers and TaqMan-MGB probes were designed and synthesized to genotype FY*A and FY*B alleles at Duffy blood group locus on a real-time PCR platform. A total of 120 samples were genotyped by using the new 5'-NA and conventional polymerase chain reaction with allele-specific primers (PCR-ASP). The results obtained by the two methods were compa

SUBMITTER: Zhou S 

PROVIDER: S-EPMC6807056 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature