Benchmarking two Saccharomyces cerevisiae laboratory strains for growth and transcriptional response to methanol.

ABSTRACT: One-carbon compounds, such as methanol, are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals, fuels, foods, and pharmaceuticals. Efficient biological production often requires extensive genetic manipulation of a microbial host strain, making well-characterised and genetically-tractable model organisms like the yeast Saccharomyces cerevisiae attractive targets for the engineering of methylotrophic metabolism. S. cerevisiae strains S288C and CEN.PK are the two best-characterised and most widely used hosts for yeast synthetic biology and metabolic engineering, yet they have unpredictable metabolic phenotypes related to their many genomic differences. We therefore sought to benchmark these two strains as potential hosts for engine

SUBMITTER: Espinosa MI 

PROVIDER: S-EPMC6807065 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature