Soluble Expression of Bladder Cancer Biomarker Matrix Metalloproteinase 1.

ABSTRACT:

Background

Matrix metalloproteinase 1 (MMP1) has been shown as a novel unique biomarker of bladder cancer in urine. MMP1 can only be detected using conventional and time-consuming methods, such as ELISA and Western. Refolded MMP1 has been achieved and used in probe screen for many years, while there is no clinical application for MMP1 detection until now. Soluble expression of MMP1 is necessary in urine detection.

Methods

cDNA of MMP1 has been isolated from human embryonic kidney 293(HEK293) cells. The catalytic domain of MMP1 is expressed as fusion protein with Escherichia coli thioredoxin (TrxA). The 30 kDa recombinant proteins were purified by Ni-chelating chromatography. The activity of soluble MMP1 was determined and compared with refolded MMP1 by zymography.

Results

SUBMITTER: Jin X 

PROVIDER: S-EPMC6807089 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature