Utility of 16S rDNA Sequencing for Identification of Rare Pathogenic Bacteria.

ABSTRACT:

Background

Phenotypic identification systems are established methods for laboratory identification of bacteria causing human infections. Here, the utility of phenotypic identification systems was compared against 16S rDNA identification method on clinical isolates obtained during a 5-year study period, with special emphasis on isolates that gave unsatisfactory identification.

Methods

One hundred and eighty-seven clinical bacteria isolates were tested with commercial phenotypic identification systems and 16S rDNA sequencing. Isolate identities determined using phenotypic identification systems and 16S rDNA sequencing were compared for similarity at genus and species level, with 16S rDNA sequencing as the reference method.

Results

Phenotypic identification systems ident

SUBMITTER: Loong SK 

PROVIDER: S-EPMC6807145 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature