MLPA Application in Clinical Diagnosis of DMD/BMD in Shanghai.

ABSTRACT:

Background

Duchenne and Becker muscular dystrophy (DMD/BMD) are X-linked recessive disorders caused by mutation in dystrophin gene. We reported 3-year clinic experience from a single hospital in Shanghai using multiplex ligation dependent probe amplification (MLPA) assay to detect DMD mutations.

Methods

Four hundred and fifty-one males and 184 females, who were clinically diagnosed as DMD/BMD patients or carriers at our hospital's outpatient clinic, were collected and performed with MLPA to detect DMD gene mutations.

Results

Seventeen novel mutation points not reported in the Leiden Muscular Dystrophy pages were identified in this study. We found that the most frequent deletion spots ranged from exon45 to exon52, and exon2, exon19 were the two most frequently detected

SUBMITTER: Ji X 

PROVIDER: S-EPMC6807150 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature