The use of oral fluid samples spotted on filter paper for the detection of measles virus using nested rt-PCR.

ABSTRACT: Measles is the leading cause of death in infants, although a vaccine is available for its prevention. At this stage of measles elimination and eradication, it is so important to confirm clinically diagnosed measles cases in the laboratory but, developing countries have troubles in collecting and maintaining the cold chain of the specimens while transporting them to the laboratories. Therefore, filter papers are good candidates for simplification of specimen collection and transportation. In this research, the effects of the temperature, at which the dried specimens were kept, and the time duration the dried specimens were kept before being tested, were studied. Since there were not enough patients' oral fluid samples available, a nested reverse transcriptase PCR (RT-PCR) that detected meas

SUBMITTER: Sheikhakbari S 

PROVIDER: S-EPMC6807562 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature