High-throughput microfluidic micropipette aspiration device to probe time-scale dependent nuclear mechanics in intact cells.

ABSTRACT: The mechanical properties of the cell nucleus are increasingly recognized as critical in many biological processes. The deformability of the nucleus determines the ability of immune and cancer cells to migrate through tissues and across endothelial cell layers, and changes to the mechanical properties of the nucleus can serve as novel biomarkers in processes such as cancer progression and stem cell differentiation. However, current techniques to measure the viscoelastic nuclear mechanical properties are often time consuming, limited to probing one cell at a time, or require expensive, highly specialized equipment. Furthermore, many current assays do not measure time-dependent properties, which are characteristic of viscoelastic materials. Here, we present an easy-to-use microfluidic device

SUBMITTER: Davidson PM 

PROVIDER: S-EPMC6810812 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature