Pluronic gel-based burrowing assay for rapid assessment of neuromuscular health in C. elegans.

ABSTRACT: Whole-organism phenotypic assays are central to the assessment of neuromuscular function and health in model organisms such as the nematode C. elegans. In this study, we report a new assay format for engaging C. elegans in burrowing that enables rapid assessment of nematode neuromuscular health. In contrast to agar environments that pose specific drawbacks for characterization of C. elegans burrowing ability, here we use the optically transparent and biocompatible Pluronic F-127 gel that transitions from liquid to gel at room temperature, enabling convenient and safe handling of animals. The burrowing assay methodology involves loading animals at the bottom of well plates, casting a liquid-phase of Pluronic on top that solidifies via a modest temperature upshift, enticing animals to reach

SUBMITTER: Lesanpezeshki L 

PROVIDER: S-EPMC6811592 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature