Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33.

ABSTRACT: Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett-Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO₄·5H₂O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH₂PO₄, 1.5 mg MgSO₄·7H₂O, 0.01 mg FeSO₄·7H₂O, 0.15 mg MnSO

SUBMITTER: Thamvithayakorn P 

PROVIDER: S-EPMC6811811 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature