Contrast bath-induced neurite outgrowth in PC12m3 cells via the p38 mitogen-activated protein kinase pathway.

ABSTRACT: We investigated the differentiation and activation of p38 MAPK induced by contrast bath in drug-hypersensitive PC12m3 mutant cells. The rate of neurite outgrowth in PC12m3 cells induced by contrast bath was much higher than that induced by warming or cooling alone or that induced by two warmings with an interval of room temperature, indicating that contrast bath has a synergistic effect. The results of an experiment using a p38 MAPK inhibitor, SB203580, showed that neurite outgrowth of PC12m3 cells induced by contrast bath is p38 MAPK-dependent. Moreover, p38 MAPK activity induced by contrast bath was greater than that induced by warming or cooling alone, indicating that the synergistic effect of a contrast bath on neurite outgrowth depends on the activity of p38 MAPK. Since calcium ions a

SUBMITTER: Motoda H 

PROVIDER: S-EPMC6812228 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature