Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.

ABSTRACT:

Objective

The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments. Standard isotope tracing experiments relying on analyses in whole cells may not accurately reflect compartmentalized metabolic processes. Analysis of compartmentalized metabolism and the dynamic interplay between compartments can potentially be achieved by stable isotope tracing followed by subcellular fractionation. Although it is recognized that metabolism can take place during biochemical fractionation of cells, a clear understanding of how such post-harvest metabolism impacts the interpretation of subcellular isotope tracing data and methods to correct for this are lacking. We set out t

SUBMITTER: Trefely S 

PROVIDER: S-EPMC6812369 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature