Establishment of a high-resolution 3D modeling system for studying pancreatic epithelial cell biology in vitro.

ABSTRACT:

Objective

Translation of basic research from bench-to-bedside relies on a better understanding of similarities and differences between mouse and human cell biology, tissue formation, and organogenesis. Thus, establishing ex vivo modeling systems of mouse and human pancreas development will help not only to understand evolutionary conserved mechanisms of differentiation and morphogenesis but also to understand pathomechanisms of disease and design strategies for tissue engineering.

Methods

Here, we established a simple and reproducible Matrigel-based three-dimensional (3D) cyst culture model system of mouse and human pancreatic progenitors (PPs) to study pancreatic epithelialization and endocrinogenesis ex vivo. In addition, we reanalyzed previously reported single-cell RNA s

SUBMITTER: Bakhti M 

PROVIDER: S-EPMC6812400 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature