Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems.

ABSTRACT: Advances in contactless acoustic liquid transfer technologies have unlocked opportunities to substantially increase the throughput of crystallization screens and decrease the consumption of reagents and consumables. Acoustic energy transfer enables crystallization experiments to be set up precisely and rapidly on a nanoliter scale. Nonetheless, adapting acoustic transfer methods to a diverse range of crystallization conditions and their physicochemical idiosyncrasies remains a major bottleneck for true universality of this technique. Even though the reagent limitations still remain an issue, we present a straightforward protocol for setting up crystallization experiments by acoustic transfer using a Labcyte Echo 550 instrument, with a focus on the technical limitations of this method, incl

SUBMITTER: Krystufek R 

PROVIDER: S-EPMC6812406 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature