The Tudor SND1 protein is an m⁶A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most abundant internal RNA modification of cellular mRNAs. m⁶A is recognised by YTH domain-containing proteins, which selectively bind to m⁶A-decorated RNAs regulating their turnover and translation. Using an m⁶A-modified hairpin present in the Kaposi's sarcoma associated herpesvirus (KSHV) ORF50 RNA, we identified seven members from the 'Royal family' as putative m⁶A readers, including SND1. RIP-seq and eCLIP analysis characterised the SND1 binding profile transcriptome-wide, revealing SND1 as an m⁶A reader. We further demonstrate that the m⁶A modification of the ORF50 RNA is critical for SND1 binding, which in turn stabilises the ORF50 transc

SUBMITTER: Baquero-Perez B 

PROVIDER: S-EPMC6812964 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature