Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii.

ABSTRACT: Enhancers are cis-regulatory elements that control most of the developmental and spatial gene expression in eukaryotes. Genetic variation of enhancer sequences is known to influence phenotypes, but the effect of enhancer variation upon enhancer functional activity and downstream phenotypes has barely been examined in any species. In the African malaria vector, Anopheles coluzzii, we identified candidate enhancers in the proximity of genes relevant for immunity, insecticide resistance, and development. The candidate enhancers were functionally validated using luciferase reporter assays, and their activity was found to be essentially independent of their physical orientation, a typical property of enhancers. All of the enhancers segregated genetically polymorphic alleles, which displayed sig

SUBMITTER: Nardini L 

PROVIDER: S-EPMC6813320 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature