Bases of Bacterial Sodium Channel Selectivity Among Organic Cations.

ABSTRACT: Hille's (1971) seminal study of organic cation selectivity of eukaryotic voltage-gated sodium channels showed a sharp size cut-off for ion permeation, such that no ion possessing a methyl group was permeant. Using the prokaryotic channel, NaChBac, we found some similarity and two peculiar differences in the selectivity profiles for small polyatomic cations. First, we identified a diverse group of minimally permeant cations for wildtype NaChBac, ranging in sizes from ammonium to guanidinium and tetramethylammonium; and second, for both ammonium and hydrazinium, the charge-conserving selectivity filter mutation (E191D) yielded substantial increases in relative permeability (P_X/P_Na). The relative permeabilities varied inversely with relative K_d calculated from

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6813354 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature