Novel peptides screened by phage display peptide library can mimic epitopes of the FnBPA-A protein and induce protective immunity against Staphylococcus aureus in mice.

ABSTRACT: Fibronectin-binding protein A (FnBPA) is a key adhesin of Staphylococcus aureus, and the protein binding to fibrinogen and elastin is mediated by its N-terminal A domain. Thus, FnBPA-A has been considered a potential vaccine candidate, but the relevant epitopes are not fully understood. Here, purified rabbit anti-FnBPA-A antibodies were produced and used to screen for peptides corresponding to or mimicking the epitope of native FnBPA-A protein by using a phage random 12-mer peptide library. After four rounds of panning, 25 randomly selected phage clones were detected by phage-ELISA and competition-inhibition ELISA. Then, eight anti-rFnBPA-A antibody-binding phage clones were selected for sequencing, and six different 12-mer peptides were displayed by these phages. Although these displayed

SUBMITTER: Li JN 

PROVIDER: S-EPMC6813446 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature