Microfluidic time-lapse analysis and reevaluation of the Bacillus subtilis cell cycle.

ABSTRACT: Recent studies taking advantage of automated single-cell time-lapse analysis have reignited interest in the bacterial cell cycle. Several studies have highlighted alternative models, such as Sizer and Adder, which differ essentially in relation to whether cells can measure their present size or their amount of growth since birth. Most of the recent work has been done with Escherichia coli. We set out to study the well-characterized Gram-positive bacterium, Bacillus subtilis, at the single-cell level, using an accurate fluorescent marker for division as well as a marker for completion of chromosome replication. Our results are consistent with the Adder model in both fast and slow growth conditions tested, and with Sizer but only at the slower growth rate. We also find that cell size variati

SUBMITTER: Lee S 

PROVIDER: S-EPMC6813450 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature