An osteogenic helioxanthin derivative suppresses the formation of bone-resorbing osteoclasts.

ABSTRACT:

Objective

The helioxanthin derivative 4-(4-methoxyphenyl)thieno[2,3-b:5,4-c']dipyridine-2-carboxamide (TH) is a low-molecular-weight compound that was identified through screening for osteogenic compounds that enhance the activity of mouse preosteoblastic MC3T3-E1 cells. In the present study, we found that TH suppressed osteoclast differentiation.

Methods

Using the hematopoietic stem cells of ddY mice, TH was added to the culture in the experimental group, and the number of osteoclasts was measured with rhodamine phalloidin staining and TRAP staining. In osteo assay, bone resorption area was compared by the von Kossa staining.

Results

Specifically, TH inhibited the cyclic guanosine monophosphate (cGMP)-degrading activity of phosphodiesterase (PDE), promoted nitric oxi

SUBMITTER: Amano H 

PROVIDER: S-EPMC6813560 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature