Mesenchymal stem cells cultured under hypoxic conditions had a greater therapeutic effect on mice with liver cirrhosis compared to those cultured under normal oxygen conditions.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) can be easily expanded. They can be acquired from medical waste such as adipose and umbilical cord tissues, are influenced by culturing conditions, and exert anti-inflammatory, antioxidant, anti-fibrotic, and angiogenic effects. We analyzed the multi-directional effects of MSCs cultured under hypoxic conditions and their underlying mechanisms in the treatment of liver cirrhosis in a mouse model.

Methods

Human bone marrow-derived MSCs cultured under hypoxic (5% O₂; hypoMSCs) and normoxic (21% O₂; norMSCs) conditions were compared by cap analysis of gene expression (CAGE) with or without serum from liver cirrhosis patients. The therapeutic effects of MSCs, including serum liver enzyme induction, fibrosis regression

SUBMITTER: Kojima Y 

PROVIDER: S-EPMC6813562 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature