Considerations and Implications in the Purification of Extracellular Vesicles - A Cautionary Tale.

ABSTRACT: Extracellular vesicles (EVs) are nano-sized particles constitutively released from cells into all biological fluids. Interestingly, these vesicles contain genetic cargoes including proteins, RNA and bioactive lipids that can be functionally delivered and affect recipient cells. As a result, there is growing interest in studying EVs in pathological conditions, including central nervous system (CNS)-related diseases, as EVs may be used for diagnostic purposes or as therapeutic agents. However, one major bottleneck is the need for better EV purification strategies when considering complex biological sources such as serum/protein-rich media or plasma. In this study, we have performed a systematic comparison study between the current gold-standard method: ultracentrifugation, to an alternative:

SUBMITTER: Lee YXF 

PROVIDER: S-EPMC6813730 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature