Engineered Cardiac Tissues Generated in the Biowire™ II: A Platform for Human-Based Drug Discovery.

ABSTRACT: Recent advances in techniques to differentiate human induced pluripotent stem cells (hiPSCs) hold the promise of an unlimited supply of human derived cardiac cells from both healthy and disease populations. That promise has been tempered by the observation that hiPSC-derived cardiomyocytes (hiPSC-CMs) typically retain a fetal-like phenotype, raising concern about the translatability of the in vitro data obtained to drug safety, discovery and development studies. The Biowire™ II platform was used to generate 3D engineered cardiac tissues (ECTs) from hiPSC-CMs and cardiac fibroblasts. Long term electrical stimulation was employed to obtain ECTs that possess a phenotype like that of adult human myocardium including a lack of spontaneous beating, the presence of a positive force-frequency resp

SUBMITTER: Feric NT 

PROVIDER: S-EPMC6813749 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature