Bioactivity-HiTES Unveils Cryptic Antibiotics Encoded in Actinomycete Bacteria.

ABSTRACT: Bacteria harbor an immense reservoir of potentially new and therapeutic small molecules in the form of "silent" biosynthetic gene clusters (BGCs). These BGCs can be identified bioinformatically but are sparingly expressed under normal laboratory growth conditions, or not at all, and therefore do not produce significant levels of the corresponding small molecule product. Several methods have been developed for activating silent BGCs. A major limitation for nearly all methods is that they require genetic procedures and/or do not report on the bioactivity of the cryptic metabolite. We herein report "Bioactivty-HiTES", an approach that links the bioactivity of cryptic metabolites to their induction while at the same time obviating the need for genetic manipulations. Using this method, we detec

SUBMITTER: Moon K 

PROVIDER: S-EPMC6813803 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature