Integration of High-Resolution Mass Spectrometry with Cryogenic Ion Vibrational Spectroscopy.

ABSTRACT: We describe an instrumental configuration for the structural characterization of fragment ions generated by collisional dissociation of peptide ions in the typical MS² scheme widely used for peptide sequencing. Structures are determined by comparing the vibrational band patterns displayed by cryogenically cooled ions with calculated spectra for candidate structural isomers. These spectra were obtained in a linear action mode by photodissociation of weakly bound D₂ molecules. This is accomplished by interfacing a Thermo Fisher Scientific Orbitrap Velos Pro to a cryogenic, triple focusing time-of-flight photofragmentation mass spectrometer (the Yale TOF spectrometer). The interface involves replacement of the Orbitrap's higher-energy collisional dissociation cell with a

SUBMITTER: Menges FS 

PROVIDER: S-EPMC6813835 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature