Proteomic alterations underlie an association with teratozoospermia in obese mice sperm.

ABSTRACT: BACKGROUND:Obesity is a worldwide crisis impairing human health. In this condition, declines in sperm quality stem from reductions in sperm concentration, motility and increase in sperm deformity. The mechanism underlying these alterations remains largely unknown. This study, determined if obesity-associated proteomic expression patterns in mice sperm parallel those in spermatozoa obtained from obese humans. METHODS:An obese mouse model was established via feeding a high-fat diet (HFD). Histological analysis identified testicular morphology and a computer assisted semen analyzer (CASA) evaluated sperm parameters. Proteome analysis was performed using a label-free quantitative LC-MS/MS system. Western blot, immunohistochemical and immunofluorescent analyses characterized protein expression

SUBMITTER: Peng Y 

PROVIDER: S-EPMC6813985 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature