Cytoplasmic delivery of functional siRNA using pH-Responsive nanoscale hydrogels.

ABSTRACT: The progress of short interfering RNA (siRNA) technologies has unlocked the development of novel alternatives for the treatment of a myriad of diseases, including viral infections, autoimmune disorders, or cancer. Nevertheless, the clinical use of these therapies faces significant challenges, mainly overcoming the charged and large nature of these molecules to effectively enter the cell. In this work, we developed a cationic polymer nanoparticle system that is able to load siRNA due to electrostatic interactions. The pH-responsiveness and membrane-disrupting ability of these carriers make them suitable intracellular delivery vehicles. In the work presented herein we synthesized, characterized, and evaluated the properties of nanoparticles based on 2-diethylaminoethyl methacrylate and tert-

SUBMITTER: Liechty WB 

PROVIDER: S-EPMC6814015 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature