Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy.

ABSTRACT:

Background

A salient topic in developmental biology relates to the molecular and genetic mechanisms that underlie tissue morphogenesis. Modern quantitative approaches to this central question frequently involve digital cellular models of the organ or tissue under study. The ovules of the model species Arabidopsis thaliana have long been established as a model system for the study of organogenesis in plants. While ovule development in Arabidopsis can be followed by a variety of different imaging techniques, no experimental strategy presently exists that enables an easy and straightforward investigation of the morphology of internal tissues of the ovule with cellular resolution.

Results

We developed a protocol for rapid and robust confocal microscopy of fixed Arabidopsi

SUBMITTER: Tofanelli R 

PROVIDER: S-EPMC6814113 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature