Water-deficit responsive microRNAs in the primary root growth zone of maize.

ABSTRACT:

Background

MicroRNA-mediated gene regulatory networks play a significant role in plant growth and development and environmental stress responses.

Results

We identified 79 microRNAs (miRNAs) and multiple miRNA variants (isomiRs) belonging to 26 miRNA families in the primary root growth zone of maize seedlings grown at one of three water potentials: well-watered (- 0.02 MPa), mild water deficit stress (- 0.3 MPa), and severe water deficit stress (- 1.6 MPa). The abundances of 3 miRNAs (mild stress) and 34 miRNAs representing 17 families (severe stress) were significantly different in water-deficit stressed relative to well-watered controls (FDR < 0.05 and validated by stem loop RT-qPCR). Degradome sequencing revealed 213 miRNA-regulated transcripts and trancriptome profiling r

SUBMITTER: Seeve CM 

PROVIDER: S-EPMC6814125 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature