Chloroplast primers for clade-wide phylogenetic studies of Thalictrum.

ABSTRACT:

Premise

Chloroplast primers were developed for phylogenetic and comparative studies in Thalictrum (Ranunculaceae).

Methods and results

We assembled and annotated the complete plastome sequence of T. thalictroides by combining multiple whole genome sequencing libraries. Using transcriptome-sequencing libraries, we also assembled a partial plastome of the related species T. hernandezii. From the newly assembled plastomes and one previously sequenced plastome, we designed and validated 28 primer pairs to target variable portions of the chloroplast genome in Thalictrum. Furthermore, we tested the validated primers in 62 species of Thalictrum. The total alignment length of the 28 regions was 15,268 bp with 2443 variable sites and 92% character o

SUBMITTER: Morales-Briones DF 

PROVIDER: S-EPMC6814179 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature