Reproducibility and Variability of Protein Analytes Measured Using a Multiplexed Modified Aptamer Assay.

ABSTRACT: BACKGROUND:There is growing interest in the use of multiplexed aptamer-based assays for large-scale proteomic studies. However, the analytic, short- and long-term variation of the measured proteins is largely uncharacterized. METHODS:We quantified 4001 plasma protein analytes from 42 participants in the Atherosclerosis Risk in Communities (ARIC) Study in split samples and at multiple visits using a multiplexed modified aptamer assay. We calculated the CV, Spearman correlation, and intraclass correlation (ICC) between split samples and evaluated the short-term (4-9 weeks) and long-term (approximately 20 years) variability using paired t-tests with log-transformed protein concentrations and Bonferroni-corrected significance thresholds. We performed principal component (PC) analysis of protei

SUBMITTER: Tin A 

PROVIDER: S-EPMC6814271 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature