Optical estimation of absolute membrane potential using fluorescence lifetime imaging.

ABSTRACT: All cells maintain ionic gradients across their plasma membranes, producing transmembrane potentials (V_mem). Mounting evidence suggests a relationship between resting V_mem and the physiology of non-excitable cells with implications in diverse areas, including cancer, cellular differentiation, and body patterning. A lack of non-invasive methods to record absolute V_mem limits our understanding of this fundamental signal. To address this need, we developed a fluorescence lifetime-based approach (VF-FLIM) to visualize and optically quantify V_mem with single-cell resolution in mammalian cell culture. Using VF-FLIM, we report V_mem distributions over thousands of cells, a 100-fold improvement relative to electrophysiological approaches. In hu

SUBMITTER: Lazzari-Dean JR 

PROVIDER: S-EPMC6814365 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature