MTORC1-Sch9 regulates hydrogen sulfide production through the transsulfuration pathway.

ABSTRACT: Endogenous hydrogen sulfide mediates anti-aging benefits of dietary restriction (DR). However, it is unclear how H₂S production is regulated by pathways related to DR. Due to the importance of mTORC1 pathway in DR, we investigated the effects of Sch9, a yeast homolog of mammalian S6K1 and a major substrate of mTORC1 on H₂S production in yeast Saccharomyces cerevisiae. We found that inhibition of the mTORC1-Sch9 pathway by SCH9 deletion, rapamycin or myriocin treatment resulted in a dramatic decrease in H₂S production. Although deficiency of SCH9 did not alter the intracellular level of methionine, the intracellular level of cysteine increased in Δsch9 cells. The expression of CYS3 and CYS4, two transsulfuration path

SUBMITTER: Lyu Z 

PROVIDER: S-EPMC6814617 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature