Differential effects of synthetic media on long-term growth, starch accumulation and transcription of ADP-glucosepyrophosphorylase subunit genes in Landoltia punctata.

ABSTRACT: Murashige & Skoog (MS) and Hoagland's media were previously used for in vitro culture of Landoltia punctata. During subsequent ex vitro culture, the use of MS medium resulted in a higher growth rate, compared to Hoagland's medium. Thus, a higher starch content of L. punctata in MS medium was previously hypothesized. Here, L. punctata strain 5632 was isolated and characterized using morphological characteristics and the atpF-atpH intergenic region. During early cultivation stage, fresh weight and relative growth rate in MS medium were lower than Hoagland's medium. Conversely, starch content in MS medium was considerably higher than in Hoagland's medium. Medium effects on expression of genes coding for starch-biosynthesis ADP-glucosepyrophosphorylase (AGPase) were determined. Genomic fragmen

SUBMITTER: Kittiwongwattana C 

PROVIDER: S-EPMC6814796 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature