Development of microsatellite markers for the Japanese endemic conifer Thuja standishii and transfer to other East Asian species.

ABSTRACT: OBJECTIVE:Design polymorphic microsatellite loci that will be useful for studies of the genetic diversity, gene-flow and reproduction in the Japanese endemic conifer Thuja standishii and test the transferability of these loci to the two other East Asian species, T. sutchuenensis and T. koraiensis. RESULTS:Fifteen loci were developed which displayed 3 to 21 alleles per locus (average?=?9.2) among 97 samples from three populations of T. standishii. Observed heterozygosity for all samples varied between 0.33 and 0.75 (average = 0.54) while expected heterozygosity values were higher with an average over the 15 loci of 0.62 (0.37-0.91). Low multi-locus probability of identity values (

SUBMITTER: Worth JRP 

PROVIDER: S-EPMC6814961 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature