Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants.

ABSTRACT: BACKGROUND:Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the research costs. Hence the present study was aimed to develop a high throughput, rapid and inexpensive method of PCR ready DNA template preparation from plant materials. METHODS:Concentration of SDS in lysis buffer, amount of starting material, period and temperature for lysis were optimized for obtaining PCR ready templates from plant materials. The method was tested using RAPD and ITS specific primers for different plant species like rice, wheat, mustard, pea, soybean, pigeonpea, tomato, maize, march lilly, bougainvillea, Indian blanket flower, nerium, petunia, purple pirouette

SUBMITTER: Choudhary P 

PROVIDER: S-EPMC6815007 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature