Ribosome collisions alter frameshifting at translational reprogramming motifs in bacterial mRNAs.

ABSTRACT: Translational frameshifting involves the repositioning of ribosomes on their messages into decoding frames that differ from those dictated during initiation. Some messenger RNAs (mRNAs) contain motifs that promote deliberate frameshifting to regulate production of the encoded proteins. The mechanisms of frameshifting have been investigated in many systems, and the resulting models generally involve single ribosomes responding to stimulator sequences in their engaged mRNAs. We discovered that the abundance of ribosomes on messages containing the IS3, dnaX, and prfB frameshift motifs significantly influences the levels of frameshifting. We show that this phenomenon results from ribosome collisions that occur during translational stalling, which can alter frameshifting in

SUBMITTER: Smith AM 

PROVIDER: S-EPMC6815119 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature