Dynamic metabolic modelling of overproduced protein secretion in Streptomyces lividans using adaptive DFBA.

ABSTRACT: BACKGROUND:Streptomyces lividans is an appealing host for the production of proteins of biotechnological interest due to its relaxed exogenous DNA restriction system and its ability to secrete proteins directly to the medium through the major Sec or the minor Tat routes. Often, protein secretion displays non-uniform time-dependent patterns. Understanding the associated metabolic changes is a crucial step to engineer protein production. Dynamic Flux Balance Analysis (DFBA) allows the study of the interactions between a modelled organism and its environment over time. Existing methods allow the specification of initial model and environment conditions, but do not allow introducing arbitrary modifications in the course of the simulation. Living organisms, however, display unexpected adaptive

SUBMITTER: Valverde JR 

PROVIDER: S-EPMC6815373 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature