Structure of the intergenic spacers in chicken ribosomal DNA.

ABSTRACT:

Background

Ribosomal DNA (rDNA) repeats are situated in the nucleolus organizer regions (NOR) of chromosomes and transcribed into rRNA for ribosome biogenesis. Thus, they are an essential component of eukaryotic genomes. rDNA repeat units consist of rRNA gene clusters that are transcribed into single pre-rRNA molecules, each separated by intergenic spacers (IGS) that contain regulatory elements for rRNA gene cluster transcription. Because of their high repeat content, rDNA sequences are usually absent from genome assemblies. In this work, we used the long-read sequencing technology to describe the chicken IGS and fill the knowledge gap on rDNA sequences of one of the key domesticated animals.

Methods

We used the long-read PacBio RSII technique to sequence the BAC clone WAG13

SUBMITTER: Dyomin A 

PROVIDER: S-EPMC6815422 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature