Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth.

ABSTRACT:

Background

As an important means of communication, exosomes play an important role in the development of hepatocellular carcinoma (HCC).

Methods

Bioinformatics analysis, dual-luciferase reporter assays, methylation-specific quantitative PCR, and ChIP-PCR analysis were used to gain insight into the underlying mechanism of miR-21 in HCC.

Results

The detection of miRNAs in exosomes of HCC showed that miR-21 expression in exosomes was positively correlated with the expression level of miR-21 in cells and negatively correlated with the expression of its target genes PTEN, PTENp1 and TETs. HCC cell-derived exosomes could increase miR-21 and p-Akt expression in HCC cells and downregulate the expression of PTEN, PTENp1 and TETs. MiR-21 inhibitors or PTENp1 overexpression vect

SUBMITTER: Cao LQ 

PROVIDER: S-EPMC6815431 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature